CAPTEURS ET BIO-CAPTEURS ÉLECTROCHIMIQUES

Ce groupe de la division  axe ses travaux de recherche sur la synthèse des molécules organiques biologiquement actives et de leurs complexes avec les éléments du groupe de platine ainsi que  la conception de capteurs ampérométriques pour la détection des molécules révélatrices de certaines maladies chroniques telles que la glycérine, le cholestérol et la sérine. L’intérêt de ce type de systèmes repose sur leurs haute sensibilité et importante limite de  la détection de la molécule objet de l’étude.

Projets

  • «Biological and Electrochemical Activity of Nanoscale Transition Metal Dendrimers and Novel Polydentate Schiff-base Complexes» : Projet de Coopération internationale (Algérie-Afrique du sud /2013-2014) :
  • « Miniaturisation de l’instrumentation analytique par la mise au point d’un dispositif micro-fluidique électrochimique (puce) pour le diagnostic  Projet à impact socio-économique DGRSDT

Top Publications

2018

Synthesis, spectral characterization, molecular modeling, antibacterial and antioxidant activities and stability study of binuclear Pd(II) and Ru(III) complexes with novel bis-[1-(2-[(2-hydroxynaphthalen-1-yl)methylidene]amino}ethyl)-1-ethyl-3-phenylthiourea] ligand: application to detection of cholesterol

A. Terbouche, C. Ait-Ramdane-Terbouche, Z. Bendjilali, H. Berriah, H. Lakhdari, D. Lerari, K. Bachari, D. Mezaoui, J.P. Guegan, D. Hauchard,  , 

Abstract

Two binuclear Pd (II) and Ir(IV) complexes with new bis [4-hydroxy-6-methyl-3-{(1E)-N-[2-(ethylamino) ethyl] ethanimidoyl}-2H-pyran-2-one] ligand (Schiff base: L) have been prepared and characterized by elemental analysis, ESI-MS, conductivity, NMR (1H NMR, 13C NMR, COSY-NMR, HMBC-NMR and HSQCNMR), EPR, FT-IR, ATR, UVeVisible spectra and cyclic voltammetry. Both antioxidant and antimicrobial activities were studied; the stability constants of the metal complexes and their interactions with glucose were estimated using spectrophotometric method. In addition, the detection of glucose using Pd(II) and Ir(IV) complexes was investigated using cavity microelectrode (CME) by chronoamperometric method. The characterization results and the molecular parameters were determined using DFT technique; a square planar geometry around Pd (II) and an octahedral geometry around Ir(IV) was found.

The antioxidant measurements showed that the ligand and its complexes exhibited remarkable activity, and the antimicrobial studies revealed that all compounds are biologically active for some bacteria and fungi. Electrochemical measurements showed the presence of sensitive responses towards glucose using both Pd(II) and Ir(IV) complexes; these results were found to be higher than other materials around glucose concentration range of 0-4mM.

2018

Synthesis, characterization and molecular modeling of binuclear Pd(II) and Ir(IV) complexes with bis [4-hydroxy-6-methyl-3-{(1E)-N-[2-(ethylamino)ethyl]ethanimidoyl}-2H-pyran-2-one]: Application to detection of glucose, Journal of Molecular Structure, 1170, (2018) 119–130.

C. Ait-Ramdane-Terbouche, A. Terbouche, M. Khalfaoui, C.L. Douib, H. Lakhdari, D. Lerari, K. Bachari, D. Mezaoui, J.P. Guegan, D. Hauchard

 Abstract

A novel bis-[1-(2-[(2-hydroxynaphthalen-1-yl) methylidene]amino}ethyl)-1-ethyl-3-phenylthiourea] Schiff base (L) and its binuclear palladium and ruthenium complexes have been prepared and characterized by ESIMS, elemental analysis, NMR (1H NMR, 13C NMR, COSY, NEOSY and HSQC), FT-IR, ATR, UV–Visible spectra, TGA measurements, conductivity and cyclic voltammetry. The experimental results and the molecular parameters calculated using DFT method revealed a square planar geometry around Pd and octahedral geometry around ruthenium metal. The antibacterial activity of the ligand L and its complexeswas evaluated against different human bacteria. In addition, the formation constants of the synthesized Schiff base-metal complexes and the systems formed with these chelates and cholesterol were estimated using spectrophotometric technique. The detection of cholesterol using novel Pd and Ru Schiff base complexes was studied using fluorometric method, and the measurements showed that the sensitive fluorometric response towards cholesterol analysis was determined using palladium complex. The limit of detection (LOD) of cholesterol calculated using this complex (4.6 μM) is lower (better) than LOD found using ruthenium complex (19.1 μM) and different compounds previously published around linear range of 0–5 mM

2016

A new electrochemical sensor based on carbon paste electrode/Ru(III) complex for determination of nitrite: Electrochemical impedance and cyclic voltammetry measurements, Measurement,92 (2016) 524-533.

Terbouche, S. Lameche, C. Ait-Ramdane-Terbouche, D. Guerniche, D. Lerari, K. Bachari, D. Hauchard

Abstract

The modified carbon paste electrode with Ru(III) complex was studied as a novel sensor for the determination of nitrite. The behavior of NO2_ at the electrode surface was investigated using electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). EIS provided useful information on the charge transfer resistance (R) at the electrode/solution. The EIS measurements showed that R is low at oxidation potentials, and decreases with increasing temperature. The increase of the constant phase element with temperature is due to the accumulation of nitrite on the electrode, thus, facilitating the electron transfer between electrode and NO2.CV was used to study the effect of pH on the electro-catalytic oxidation of NO2_ and to determine the limit of detection (LOD). CV measurements showed a good linear relationship between the oxidation current and the concentration of NO2 _ over a wide concentration range 0–1.38 _ 10_2 mol L_1. Low detection limit of 1.39 _ 10_6 mol L_1 toward NO2 _ was obtained. LOD decreased by 23.2% compared to that determined using cavity microelectrode.

A new ternary ruthenium(III) complex with 1,3-bis(salicylideneamino) propan-2-ol and 3-picolylamine: Synthesis, characterization, density functional theory and preparation of electrochemical sensor for nitrite analysis, Journal of Molecular Structure, 1076 (2014) 501–511.A.

Terbouche, C. Ait-Ramdane-Terbouche, S. Djebbar, D. Guerniche,R. Bagtache, N.E.H. Bensiradj, A. Saal, D. Hauchard,

Abstract

A novel electrochemical sensor based on graphite (G) functionalised with a new ternary ruthenium(III) complex was developed and applied to detect nitrite in aqueous solution. The Ru(III) complex was synthesized using 1,3-bis(salicylideneamino) propan-2-ol polydentate Schiff base (BSAP) and 3-Picolylamine (PLA), and was characterized by elemental analysis, Fourier transform infrared spectroscopy (FT-IR), Ultraviolet–visible spectrophotometry (UV–Visible), gradient-assisted hetero nuclear single quantum coherence spectroscopy (gHSQC) and cyclic voltammetry technique. In addition, the structure of the synthesized complex was optimized using density functional theory (DFT). The results showed that the ternary Ru(III)–BSAP–PLA complex was formed and the adapted structure was an tetrahedral geometry. The electrochemical behavior of nitrite at the sensor prepared using G/Ru(III)–BSAP–PLA composite shows that the evaluated electron transfer coefficient (a = 0.83) indicates a very significant electrocatalytic mechanism for oxidation of nitrite in the presence of the Ru(III)–BSAP–PLA complex.

Electrochemical sensors for the detection of nitrite ions in water using modified electrode based on copper complexes synthesised with Algerian humic acid modified with ethylenediamine and triethylenetetramine,  Talanta,119 (2014) 214–225.

C. Ait Ramdane-Terbouche, A. Terbouche, S. Djebbar, D. Hauchard,  

Abstract

The response and efficiency of new sensors for nitrite ions analysis have been studied electrochemically. These sensors were developed by modifying a carbon paste electrode (CPE) with copper (II) complexes formed with commercial (PFHA) and Algerian (YHA) humic acids and their modified compounds with ethylenediamine (EDA) or triethylenetetramine (TETA). The developed mechanism is based on the electrochemical oxidation of NO2on the modified CPE for different nitrite concentrations. The obtained results showed that the carbon paste electrode modified with copper (II)-modified humic acids complexes (Cu-MHA) exhibited substantial electrocatalytic effect on the oxidation of nitrite anions compared with carbon paste electrode. The sensitivity of the modified CPE towards nitrite concentrations depends on the nature of the humic acid and its modified compounds. The measurements performed by using CPE/Cu-YHA-EDA and CPE/Cu-YHA-TETA gave the best sensitivity and a good linear response of current versus nitrite concentrations. The oxidation peak current of nitrite at CPE/Cu-YHA-TETA and CPE/Cu-YHA-EDA electrodes in weak acid solution is proportional to the concentration of nitrite over the range 0-1.38×10-2 mol L-1 with a limit of detection ( LOD ) of 1.46 μmol L-1 ( Sensitivity = 41.06μA mmol L-1 ) and 2.17 μmol L-1 (Sensitivity = 27.63μA mmol L-1 ), respectively. Compared to the sensors published in the literature, our CPE/Cu-YHA-TETA and CPE/Cu-YHA-EDA electrodes exhibit a good catalytic activity towards nitrite oxidation and a low limit of detection over a wide nitrite concentrations range.