M. AISSAOUI, M. RAHMOUN, H. LATRACHE, S. BAREK, A. ELASSRI, C. BENSOUICI, A-I. EL HACI, N. CHOUKCHOU-BRAHAM,

Published online: 03 Aug 2020

Journal of Complementary and Integrative Medicine | Ahead of Print

Abstract

Objectives

In order to explore Coffea canephora green seeds as natural extract for application in the functional-food industry, we focused this study to the evaluation of the antioxidant and the antiadhesion effect of C. canephora green seeds extracts.

Methods

The analysis of C. canephora green seeds extracts was carried out by RP-HPLC-PDA. These extracts were screened for antioxidant activities by ABTS and phenanthroline assays. The antibacterial activity was determined by microdilution method against three reference bacteria. The inhibition of bacterial adhesion at 1/8 MIC was carried out against three reference bacteria.

Results

The RP-HPLC-PDA revealed the presence of gallic acid, vanillin, quercetin, chlorogenic acid, and P-coumaric acid. The n-buatnol extract have the highest activity ABTS assays (3.96 ± 0.08 μg/mL). For this extract, the A0.5 was 1.90 ± 0.05 μg/mL for phenanthroline assay. The n-butanol extract and the methanolic extract have the higher antibacterial activity against Staphylococcus aureus ATCC 25923 (40 µg/mL).At MIC/8, the extracts of C. canephora showed 70% higher antidhesive activity against S. aureus ATCC 25923.

Conclusions

Our finding provides an effective and specific new approach to the search of antioxidant and antiadhesive compounds for different uses.

Keywords: antioxidant activityantibacterial activityadhesionCoffea canephora green seedsRP-HPLC-PDA

https://www.degruyter.com/view/journals/jcim/ahead-of-print/article-10.1515-jcim-2020-0046/article-10.1515-jcim-2020-0046.xml